Exhibiting at Fi South America 2025

Zhongnuo Biotechnology Development Jiangsu Co., Ltd., established in 2009 and headquartered in Yancheng, Jiangsu Province, China, is a specialized high-tech enterprise focused on the research, production, and global supply of food-grade enzymes. With a production facility covering over 12,000 square meters and a technical team dedicated to enzyme biotechnology, Zhongnuo has grown into one of China’s most prominent manufacturers of high-activity, stable, and application-specific enzymes used across the food, beverage, pharmaceutical, and fermentation industries. ## Core Business and Product Portfolio Zhongnuo’s portfolio consists of a wide range of functional enzymes that play critical roles in food processing, fermentation enhancement, and industrial applications: - Food Enzymes: - Acid Lactase (β-Galactosidase) – Used for lactose hydrolysis in dairy and infant nutrition products. - Invertase (Saccharase) – Converts sucrose into glucose and fructose in confectionery and beverages. - Dextranase (α-Glucanase) – Breaks down dextrans in sugar processing and wine fermentation. - Inulinase – Converts inulin to fructose; used in functional sweetener production. - Glucose Oxidase – Oxygen scavenger in bakery and egg processing. - Chymosin (Rennin) – Coagulates milk proteins in cheese-making. - Nattokinase – Functional enzyme for cardiovascular health in dietary supplements. - Fructosyltransferase – For prebiotic oligosaccharide production. - Lipase – Used in baking and flavor development in dairy and meat. These enzymes are available in powder and liquid forms, with varying activity units customized according to industry-specific requirements. ## Specialization and Unique Strengths - High-Activity Enzyme Formulation: Each product is engineered for high purity and high specific activity to reduce dosage and cost in end-use. - Tailored Applications: Customized solutions for industries such as baking, brewing, dairy, and functional nutrition. - Dual Automated Production Lines: Ensure uninterrupted, high-volume manufacturing with strict process control and batch consistency. - R&D and Fermentation Expertise: In-house strain improvement, fermentation optimization, and downstream purification under GMP-compliant protocols. - Environmentally Friendly: Adheres to green manufacturing principles using microbial fermentation without harmful solvents. ## Financials While financials are not publicly disclosed, multiple industry listings and trade platform metrics indicate strong operational performance: - Estimated Annual Revenue (2024): USD 10–14 million - Annual Production Capacity: Over 1,500 metric tons of enzymes - Export Share: Estimated at 65% of total sales - R&D Investment: Approx. 7% of revenue, focused on enzyme innovation and formulation science ## Shipment Records According to data from Volza, Trademo, and Alibaba Trade Records: - Over 1,300 international shipments since 2020 - Primary exported products: β-Galactosidase, Invertase, Glucose Oxidase - Export destinations include: India, Turkey, USA, Brazil, Thailand, Pakistan - Key sectors: Food and Beverage Manufacturing, Nutraceuticals, and Biotech Fermentation Companies Bulk enzyme shipments typically range from 200 kg to 2,000 kg per order, customized in packaging formats from 25 kg drums to 1 kg foil bags. ## Target Market Zhongnuo targets a broad range of industries and clients: - Dairy & Ice Cream Producers - Bakeries and Confectionery Manufacturers - Functional Food & Beverage Companies - Nutraceutical & Supplement Brands - Fermentation Processors - Industrial Enzyme Distributors & Traders Its enzyme solutions are also in demand from companies seeking clean-label, plant-based, and allergen-free functional processing agents. ## Manufacturing Capabilities The company’s production and quality assurance systems are built to deliver scale and precision: - Fermentation Tanks with 5,000L and 10,000L capacity - Downstream Purification Units: Including microfiltration, ultrafiltration, and vacuum drying - Spray Drying Systems for powder enzyme manufacturing - QC Laboratory equipped with UV-Vis Spectrophotometry, HPLC, and Enzymatic Activity Assay Systems - Automated Filling and Packing Units for diverse customer packaging requirements Key production capacities: - Lactase: 600+ tons/year - Invertase & Glucose Oxidase: 400+ tons/year combined - Functional Enzymes (Nattokinase, Chymosin, etc.): 300+ tons/year ## Certifications and Compliance Zhongnuo ensures strict adherence to international quality and food safety standards: - FSSC 22000 – Food Safety System Certification - ISO 22000:2018 – Food Safety Management - ISO 9001:2015 – Quality Management System - HALAL & KOSHER Certified Ingredients - China National Food Safety Standards (GB) - Third-Party Testing Reports from SGS and Intertek available on request Each product is shipped with detailed COA, MSDS, enzyme activity specification sheet, and microbial testing data. ## Customer Feedback Global buyers have recognized the company's product quality and support: > “Consistently high activity and no off-odor. Zhongnuo’s lactase improved our yogurt texture and sweetness without added sugar.” > — R&D Lead, Dairy Manufacturer, Thailand > “Prompt shipment, well-documented technical files, and great customer service. Their invertase meets our confectionery line expectations.” > — Purchasing Manager, Candy Manufacturer, Brazil ## Achievements and Recognition - Top 10 Emerging Enzyme Exporters in China (2023, Food Ingredients Online China Rankings) - Approved OEM enzyme supplier to private-label supplement brands in Southeast Asia - Long-term exhibitor at CPhI China, Fi Asia, and FoodTech China - Developed high-stability enzyme formulations for tropical markets (2022–2023) - Certified by China Food Additives and Ingredients Association (CFAA) for GMP-compliant manufacturing Zhongnuo Biotechnology Development Jiangsu Co., Ltd. continues to advance the field of enzyme-based solutions by combining precision fermentation, customer-centered formulation, and a global export network, reinforcing its position as a key supplier to the evolving needs of the food and health industries.